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fluorescent isothiocyanate labeled lectin  (Vector Laboratories)


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    Vector Laboratories fluorescent isothiocyanate labeled lectin
    Figure 1. Comparison of the current and new methods for laser <t>microdissection–lectin</t> microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.
    Fluorescent Isothiocyanate Labeled Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+isothiocyanate+labeled+lectin/pm30736315-215-36-47?v=Vector+Laboratories
    Average 95 stars, based on 363 article reviews
    fluorescent isothiocyanate labeled lectin - by Bioz Stars, 2026-07
    95/100 stars

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    1) Product Images from "An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection."

    Article Title: An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms20030700

    Figure 1. Comparison of the current and new methods for laser microdissection–lectin microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.
    Figure Legend Snippet: Figure 1. Comparison of the current and new methods for laser microdissection–lectin microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.

    Techniques Used: Comparison, Laser Capture Microdissection, Microarray, Dissection, Staining



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    Figure 1. Comparison of the current and new methods for laser <t>microdissection–lectin</t> microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.
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    Figure 1. Comparison of the current and new methods for laser microdissection–lectin microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.

    Journal: International journal of molecular sciences

    Article Title: An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection.

    doi: 10.3390/ijms20030700

    Figure Lengend Snippet: Figure 1. Comparison of the current and new methods for laser microdissection–lectin microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.

    Article Snippet: For fluorescent double staining, after blocking as described above, the sections were serially incubated with a biotinylated probe (5 μg/mL in PBS; PNA, anti-CK5 antibody, and DBA from Vector Laboratories) for 5 h at RT, a fluorescent isothiocyanate-labeled lectin (5 μg/mL in PBS; UEA-I, PNA, DBA from Vector Laboratories) overnight at 4 ◦C, and Alexa Fluor 647-conjugated streptavidin (1 μg/mL in PBS; Invitrogen), and then mounted in ProLong Gold antifade reagent (Invitrogen).

    Techniques: Comparison, Laser Capture Microdissection, Microarray, Dissection, Staining